|
KMID : 0364819850230040241
|
|
Korean Journal of Microbiology
1985 Volume.23 No. 4 p.241 ~ p.247
|
|
|
Purification and Characterization of the Regulatory Substance of Furfural Biodegradation in Pseudomonas fluorescens
|
|
|
|
|
Abstract
|
|
|
1
The objectives of this study were to isolate and identify ninhydrin positive substance(s) produced in the culture broth of Pseudomonas fluorescens. It was found that the NPS could stimulate bioconversion of furfural into furoic acid. In order to isolate the NPS from the culture broth, cell free filtrate was subjected to ion-exchange chromatography, gel-permeation and finally to cellulose column chromatography. The purified NPS was white amorphous powder and very soluble in water, slightly soluble in methanol and very insoluble in organic solvents. UV, and IR absorption spectra. 1H and
13C-NMR were measured in order to identify the chemical structure of the NPS.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|